diff --git a/python/mozbuild/mozbuild/build_commands.py b/python/mozbuild/mozbuild/build_commands.py
--- a/python/mozbuild/mozbuild/build_commands.py
+++ b/python/mozbuild/mozbuild/build_commands.py
@@ -183,10 +183,11 @@
             directory=directory,
             verbose=verbose,
             keep_going=keep_going,
             mach_context=command_context._mach_context,
             append_env=append_env,
+            virtualenv_topobjdir=orig_topobjdir,
         )
         if status != 0:
             return status
 
         # Packaging the instrumented build is required to get the jarlog
@@ -206,11 +207,11 @@
             pgo_env["LLVM_PROFDATA"] = instr.config_environment.substs.get(
                 "LLVM_PROFDATA"
             )
         pgo_env["JARLOG_FILE"] = mozpath.join(orig_topobjdir, "jarlog/en-US.log")
         pgo_cmd = [
-            instr.virtualenv_manager.python_path,
+            command_context.virtualenv_manager.python_path,
             mozpath.join(command_context.topsrcdir, "build/pgo/profileserver.py"),
         ]
         subprocess.check_call(pgo_cmd, cwd=instr.topobjdir, env=pgo_env)
 
         # Set the default build to MOZ_PROFILE_USE
diff --git a/python/mozbuild/mozbuild/controller/building.py b/python/mozbuild/mozbuild/controller/building.py
--- a/python/mozbuild/mozbuild/controller/building.py
+++ b/python/mozbuild/mozbuild/controller/building.py
@@ -1220,10 +1220,11 @@
         directory=None,
         verbose=False,
         keep_going=False,
         mach_context=None,
         append_env=None,
+        virtualenv_topobjdir=None,
     ):
         """Invoke the build backend.
 
         ``what`` defines the thing to build. If not defined, the default
         target is used.
@@ -1297,10 +1298,11 @@
                 config_rc = self.configure(
                     metrics,
                     buildstatus_messages=True,
                     line_handler=output.on_line,
                     append_env=append_env,
+                    virtualenv_topobjdir=virtualenv_topobjdir,
                 )
 
                 if config_rc != 0:
                     return config_rc
 
@@ -1635,10 +1637,11 @@
         metrics,
         options=None,
         buildstatus_messages=False,
         line_handler=None,
         append_env=None,
+        virtualenv_topobjdir=None,
     ):
         # Disable indexing in objdir because it is not necessary and can slow
         # down builds.
         self.metrics = metrics
         mkdir(self.topobjdir, not_indexed=True)
@@ -1658,15 +1661,16 @@
             if line.startswith("export "):
                 k, eq, v = line[len("export ") :].partition("=")
                 if eq == "=":
                     append_env[k] = v
 
+        virtualenv_topobjdir = virtualenv_topobjdir or self.topobjdir
         build_site = CommandSiteManager.from_environment(
             self.topsrcdir,
             lambda: get_state_dir(specific_to_topsrcdir=True, topsrcdir=self.topsrcdir),
             "build",
-            os.path.join(self.topobjdir, "_virtualenvs"),
+            os.path.join(virtualenv_topobjdir, "_virtualenvs"),
         )
         build_site.ensure()
 
         command = [build_site.python_path, os.path.join(self.topsrcdir, "configure.py")]
         if options: